Overview

  • Product name

    Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08]
    See all Tyrosyl tRNA synthetase/TyrRS primary antibodies
  • Description

    Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase/TyrRS
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ICC, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Tyrosyl tRNA synthetase/TyrRS.

  • Positive control

    • HeLa cells and cell lysate and NIH3T3 cell lysate.
  • General notes

    Previously labelled as Tyrosyl tRNA synthetase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality

    Monoclonal
  • Clone number

    YAR5H08
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 20 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • Involvement in disease

    Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities

    Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Images

  • Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Anti-Tyrosyl tRNA synthetase/TyrRS antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

References

This product has been referenced in:

  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

Should you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up