Anti-TZFP/ZBTB32 antibody (ab235306)
Key features and details
- Rabbit polyclonal to TZFP/ZBTB32
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TZFP/ZBTB32 antibody -
Description
Rabbit polyclonal to TZFP/ZBTB32 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee -
Immunogen
Recombinant fragment corresponding to Human TZFP/ZBTB32 aa 1-300.
Database link: Q9Y2Y4 -
Positive control
- WB: Mouse brain tissue lysate; Jurkat whole cell lysate (ab7899). IHC-P: Human adrenal gland and testis tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 53 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 53 kDa. |
Target
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Function
DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells. -
Tissue specificity
Predominantly expressed in testis. Some isoforms are ubiquitously expressed. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers. -
Domain
The C-terminal zinc finger domain functions as a transcriptional transactivator.
The BTB (POZ) domain possesses repressor activity. -
Cellular localization
Nucleus. Located in nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 745538 Chimpanzee
- Entrez Gene: 27033 Human
- Entrez Gene: 58206 Mouse
- Omim: 605859 Human
- SwissProt: A2T7E6 Chimpanzee
- SwissProt: Q9Y2Y4 Human
- SwissProt: Q9JKD9 Mouse
- Unigene: 99430 Human
see all -
Alternative names
- FANCC interacting protein antibody
- FANCC-interacting protein antibody
- Fanconi anemia zinc finger protein antibody
see all
Images
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All lanes : Anti-TZFP/ZBTB32 antibody (ab235306) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 53 kDa -
Paraffin-embedded human adrenal gland tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235306 has not yet been referenced specifically in any publications.