Key features and details
- Rabbit polyclonal to TZFP/ZBTB32
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TZFP/ZBTB32 antibody
DescriptionRabbit polyclonal to TZFP/ZBTB32
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee
Recombinant fragment corresponding to Human TZFP/ZBTB32 aa 1-294.
MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGV SQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARAL GVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQK PEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPV GQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWA EAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQR
Database link: Q9Y2Y4
- WB: Mouse brain tissue lysate; Jurkat whole cell lysate (ab7899). IHC-P: Human adrenal gland and testis tissues.
This product was previously labelled as TZFP
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 53 kDa.|
FunctionDNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
Tissue specificityPredominantly expressed in testis. Some isoforms are ubiquitously expressed.
Sequence similaritiesBelongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers.
DomainThe C-terminal zinc finger domain functions as a transcriptional transactivator.
The BTB (POZ) domain possesses repressor activity.
Cellular localizationNucleus. Located in nuclear speckles.
- Information by UniProt
- FANCC interacting protein antibody
- FANCC-interacting protein antibody
- Fanconi anemia zinc finger protein antibody
All lanes : Anti-TZFP/ZBTB32 antibody (ab235306) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 53 kDa
Paraffin-embedded human adrenal gland tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235306 has not yet been referenced specifically in any publications.