Overview

  • Product name

    Anti-TZFP/ZBTB32 antibody
  • Description

    Rabbit polyclonal to TZFP/ZBTB32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment corresponding to Human TZFP/ZBTB32 aa 1-294.
    Sequence:

    MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGV SQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARAL GVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQK PEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPV GQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWA EAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQR


    Database link: Q9Y2Y4

  • Positive control

    • WB: Mouse brain tissue lysate; Jurkat whole cell lysate (ab7899). IHC-P: Human adrenal gland and testis tissues.
  • General notes

     This product was previously labelled as TZFP

     

Properties

Applications

Our Abpromise guarantee covers the use of ab235306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 53 kDa.

Target

  • Function

    DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • Tissue specificity

    Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • Domain

    The C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • Cellular localization

    Nucleus. Located in nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Images

  • All lanes : Anti-TZFP/ZBTB32 antibody (ab235306) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 53 kDa

  • Paraffin-embedded human adrenal gland tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human testis tissue stained for TZFP/ZBTB32 using ab235306 at 1/100 dilution in immunohistochemical analysis.

References

ab235306 has not yet been referenced specifically in any publications.

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