U-87 MG nuclear extract lysate (ab14903)
Overview
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Product name
U-87 MG nuclear extract lysate -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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Research areas
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Background
Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab14903 has not yet been referenced specifically in any publications.