U-87 MG nuclear extract lysate (ab14903)

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Overview

  • Product name

    U-87 MG nuclear extract lysate

  • General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). 

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • Research areas

  • Background

    Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14903? Please let us know so that we can cite the reference in this datasheet.

ab14903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Hi,
I'm a student and work in a lab. Referenced in the subject line is the nuclear lysate, but just out of curiosity, I was wondering if you have vials of U-87 MG cellular lysate and if so, how many vials of it you sell per month. Thank you for your help.

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Answer

Thank you for your inquiry.

Unfortunately, I am sorry to confirm we do not sell U-87 MG whole cell lysate, only the nuclear lysate (ab14903).

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:

http://www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

I'm sorry we do not have the specific product for your requirements on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Question

Email: kaml@labcorp.com I am interested in the U-87 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate product to isolate total RNA that is stable. Do you know if RNA is stable in your lysate, and if not, is it possible to get whole live cells in order to isolate RNA?

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Answer

Thank you for your interest in ab14903. This lysate is a protein lysate only and is not suitable for DNA/RNA work. Unfortunately we do not have cell lines or tissues available for RNA isolation on our catalog. I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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