U0126, Selective MKK inhibitor (ab120241)
Key features and details
- Selective MKK inhibitor
- CAS Number: 109511-58-2
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
U0126, Selective MKK inhibitor -
Description
Selective MKK inhibitor -
Biological description
Selective non-competitive inhibitor of MAP kinase kinase (MKK). Inhibits MKK1, MKK2 (IC50 values of 0.07 and 0.06 μM, respectively) and MKK5 with little or no effect on a wide range of other kinases. Centrally active following systemic administration in vivo.
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CAS Number
109511-58-2 -
Chemical structure
Properties
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Chemical name
1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene -
Molecular weight
380.49 -
Molecular formula
C18H16N6S2 -
PubChem identifier
3006531 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
N/C(Sc1ccccc1N)=C(/C#N)C(C#N)=C(/N)Sc2ccccc2N -
Source
Synthetic
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Research areas
Images
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Chemical Structure - U0126, Selective MKK inhibitor (ab120241)2D chemical structure image of ab120241, U0126, Selective MKK inhibitor
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Western blot - U0126, Selective MKK inhibitor (ab120241)Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
Performed under reducing conditions; Samples l
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (16)
ab120241 has been referenced in 16 publications.
- Sugita J et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun 12:1910 (2021). PubMed: 33771995
- Hamon A et al. Linking YAP to Müller Glia Quiescence Exit in the Degenerative Retina. Cell Rep 27:1712-1725.e6 (2019). PubMed: 31067458
- Ahmed SBM et al. Studying the ShcD and ERK interaction under acute oxidative stress conditions in melanoma cells. Int J Biochem Cell Biol 112:123-133 (2019). PubMed: 31121283
- Niu C et al. Metformin alleviates hyperglycemia-induced endothelial impairment by downregulating autophagy via the Hedgehog pathway. Autophagy 15:843-870 (2019). PubMed: 30653446
- Li X et al. The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network. Oxid Med Cell Longev 2019:9706792 (2019). PubMed: 30911355