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    u0126-selective-mkk-inhibitor-ab120241.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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U0126, Selective MKK inhibitor (ab120241)

  • Datasheet
  • SDS
  • COA
Submit a review Submit a question References (18)

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Chemical Structure - U0126, Selective MKK inhibitor (ab120241)
  • Western blot - U0126, Selective MKK inhibitor (ab120241)

Key features and details

  • Selective MKK inhibitor
  • CAS Number: 109511-58-2
  • Soluble in DMSO to 100 mM
  • Form / State: Solid
  • Source: Synthetic

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Overview

  • Product name

    U0126, Selective MKK inhibitor
  • Description

    Selective MKK inhibitor
  • Biological description

    Selective non-competitive inhibitor of MAP kinase kinase (MKK). Inhibits MKK1, MKK2 (IC50 values of 0.07 and 0.06 μM, respectively) and MKK5 with little or no effect on a wide range of other kinases. Centrally active following systemic administration in vivo.

  • CAS Number

    109511-58-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene
  • Molecular weight

    380.49
  • Molecular formula

    C18H16N6S2
  • PubChem identifier

    3006531
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    N/C(Sc1ccccc1N)=C(/C#N)C(C#N)=C(/N)Sc2ccccc2N
  • Source

    Synthetic

  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
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    • Pharmacology
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    • Kinase
    • MAPK
    • MKK/MEK
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Signal transduction
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    • MKK/MEK
    • Inhibitors
    • Biochemicals
    • Research Area
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Images

  • Chemical Structure - U0126, Selective MKK inhibitor (ab120241)
    Chemical Structure - U0126, Selective MKK inhibitor (ab120241)
    2D chemical structure image of ab120241, U0126, Selective MKK inhibitor
  • Western blot - U0126, Selective MKK inhibitor (ab120241)
    Western blot - U0126, Selective MKK inhibitor (ab120241)
    Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

    Lane 1: Marker
    Lane 2: A431 lysate starved
    Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
    Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
    Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

    Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

    Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

    Observed bands:
    RSK1p90 (pS380) band size: 83 kDa
    AKT1 (pS473) band size: 56 kDa
    ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
    RPS6 (pS235/236) band size: 31 kDa
    Rab11a band size: 24 kDa
    Unknown band: 130kDa
    Performed under reducing conditions; Samples l

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (18)

Publishing research using ab120241? Please let us know so that we can cite the reference in this datasheet.

ab120241 has been referenced in 18 publications.

  • Sugita J  et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun 12:1910 (2021). PubMed: 33771995
  • van Krieken PP  et al. Oncostatin M suppresses browning of white adipocytes via gp130-STAT3 signaling. Mol Metab 54:101341 (2021). PubMed: 34547509
  • Lan CN  et al. MAPK inhibitors protect against early-stage osteoarthritis by activating autophagy. Mol Med Rep 24:N/A (2021). PubMed: 34590154
  • Hamon A  et al. Linking YAP to Müller Glia Quiescence Exit in the Degenerative Retina. Cell Rep 27:1712-1725.e6 (2019). PubMed: 31067458
  • Ahmed SBM  et al. Studying the ShcD and ERK interaction under acute oxidative stress conditions in melanoma cells. Int J Biochem Cell Biol 112:123-133 (2019). PubMed: 31121283
View all Publications for this product

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