Overview

  • Product name

    Anti-U1-C antibody [4H12]
    See all U1-C primary antibodies
  • Description

    Rat monoclonal [4H12] to U1-C
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Mouse U1-C.

  • Positive control

    • L929 cells; HeLa total cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purification notes

    ab122901 was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by a propriety chromatography processes and filter sterilized.
  • Clonality

    Monoclonal
  • Clone number

    4H12
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Function

    This protein is associated with snRNP U1.
  • Sequence similarities

    Belongs to the U1 small nuclear ribonucleoprotein C family.
    Contains 1 matrin-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ20302 antibody
    • OTTHUMP00000016238 antibody
    • OTTMUSP00000026897 antibody
    • RU1C_HUMAN antibody
    • Small nuclear ribonucleoprotein polypeptide C antibody
    • Snrpc antibody
    • U1 C antibody
    • U1 small nuclear ribonucleoprotein 1C antibody
    • U1 small nuclear ribonucleoprotein C antibody
    • U1 small nuclear RNP specific C antibody
    • U1 snRNP C antibody
    • U1 snRNP protein C antibody
    • U1-C antibody
    • U1C antibody
    • U1C protein antibody
    • Yhc1 antibody
    see all

Images

  • Anti-U1-C antibody [4H12] (ab122901) + HeLa total cell extract.

    Predicted band size: 17 kDa

  • ab122901 staining U1-C in Mouse L929 cells by Immunoflourescence (green). Right image shows the cells DNA stained with Hoechst.

  • Immunoprecipitation of U1-C with ab122901.
    Lane 1: no extract.
    Lane 2: HeLa total cell extract.
    HL and LC: heavy and light chains.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NSC34 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Loading amount
10 µg
Specification
NSC34 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 23 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up