Key features and details
- Rat monoclonal [4H12] to U1-C
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG2a
Product nameAnti-U1-C antibody [4H12]
See all U1-C primary antibodies
DescriptionRat monoclonal [4H12] to U1-C
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Monkey
Recombinant full length protein corresponding to Mouse U1-C.
- L929 cells; HeLa total cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purification notesab122901 was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by a propriety chromatography processes and filter sterilized.
Light chain typekappa
Our Abpromise guarantee covers the use of ab122901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionThis protein is associated with snRNP U1.
Sequence similaritiesBelongs to the U1 small nuclear ribonucleoprotein C family.
Contains 1 matrin-type zinc finger.
- Information by UniProt
- FLJ20302 antibody
- OTTHUMP00000016238 antibody
- OTTMUSP00000026897 antibody
Anti-U1-C antibody [4H12] (ab122901) + HeLa total cell extract.
Predicted band size: 17 kDa
ab122901 staining U1-C in Mouse L929 cells by Immunoflourescence (green). Right image shows the cells DNA stained with Hoechst.
Immunoprecipitation of U1-C with ab122901.
Lane 1: no extract.
Lane 2: HeLa total cell extract.
HL and LC: heavy and light chains.
ab122901 has been referenced in 1 publication.
- Rogalska ME et al. Therapeutic activity of modified U1 core spliceosomal particles. Nat Commun 7:11168 (2016). PubMed: 27041075