Key features and details
- Rabbit polyclonal to U1-C
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-U1-C antibody
See all U1-C primary antibodies
DescriptionRabbit polyclonal to U1-C
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Rhesus monkey, Xenopus tropicalis
- Human testis tissue; RT-4 and U-251 MG cell lysates; MCF7 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 17 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionThis protein is associated with snRNP U1.
Sequence similaritiesBelongs to the U1 small nuclear ribonucleoprotein C family.
Contains 1 matrin-type zinc finger.
- Information by UniProt
- FLJ20302 antibody
- OTTHUMP00000016238 antibody
- OTTMUSP00000026897 antibody
All lanes : Anti-U1-C antibody (ab204504) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Developed using the ECL technique.
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling U1-C with ab204504 at 1/200 dilution.
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling U1-C with ab204504 at 4 µg/mL (green).
ab204504 has not yet been referenced specifically in any publications.