Overview

  • Product name

  • Description

    Rabbit polyclonal to U1-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Rhesus monkey, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human U1-C aa 34-80.
    Sequence:

    VKDYYQKWMEEQAQSLIDKTTAAFQQGKIPPTPFSAPPPAGAMIPPP


    Database link: P09234

  • Positive control

    • Human testis tissue; RT-4 and U-251 MG cell lysates; MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 17 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

Images

  • All lanes : Anti-U1-C antibody (ab204504) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling U1-C with ab204504 at 1/200 dilution.

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling U1-C with ab204504 at 4 µg/mL (green).

References

ab204504 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204504.
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