Overview

  • Product name

    Anti-U1-C antibody [EPR16034]
    See all U1-C primary antibodies
  • Description

    Rabbit monoclonal [EPR16034] to U1-C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human U1-C aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P09234

  • Positive control

    • K562, Jurkat, HeLa, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates: Human tonsil tissue, Human squamous cell carcinoma of cervix tissue and Rat liver tissue; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16034
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/40.
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-U1-C antibody [EPR16034] (ab192028) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-U1-C antibody [EPR16034] (ab192028) at 1/10000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

  • Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling U1-C with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised HeLa cells labeling U1-C with ab192028 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution (green). The nuclear counter stain is Dapi (blue). 

    The two negative controls are ab192028 at 1/500 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.

  • Western blot analysis of U1-C in K562 lysate immunoprecipitated using ab192028 at 1/40 dilution (Lane 1).  Lane 2: PBS instead of K562 lysate.

     

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Subramania S  et al. SAM68 interaction with U1A modulates U1 snRNP recruitment and regulates mTor pre-mRNA splicing. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30767021) »
  • Královicová J  et al. PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM. Nucleic Acids Res 46:6166-6187 (2018). Read more (PubMed: 29788428) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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