Key features and details
- Rabbit polyclonal to U1A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-U1A antibody
See all U1A primary antibodies
DescriptionRabbit polyclonal to U1A
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within amino acids 139-282 of Human U1A (Uniprot ID: P09012).
- A549, HeLa and HepG2 whole cell lysates; HeLa cells; Mouse liver tissue and Human breast carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 31 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionBinds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
Sequence similaritiesBelongs to the RRM U1 A/B'' family.
Contains 2 RRM (RNA recognition motif) domains.
- Information by UniProt
- fc19d01 antibody
- Mud1 antibody
- small nuclear ribonucleoprotein polypeptide A antibody
All lanes : Anti-U1A antibody (ab155054) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
Confocal Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling U1A with ab155054 at 1/500 dilution (green). Alpha-tubulin filaments are labeled red.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling U1A with ab155054 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling U1A with ab155054 at 1/250 dilution.
ab155054 has been referenced in 2 publications.
- Doggett K et al. Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice. RNA 24:1856-1870 (2018). PubMed: 30254136
- Soboleva TA et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . PubMed: 28234895