Overview

  • Product name
  • Description
    Mouse polyclonal to U1A
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-282 of Human U1A (NP_004587.1).

  • Positive control
    • HeLa cells. U1A transfected 293T cell line. Human liver tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
  • Sequence similarities
    Belongs to the RRM U1 A/B'' family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • fc19d01 antibody
    • Mud1 antibody
    • small nuclear ribonucleoprotein polypeptide A antibody
    • snRNP A antibody
    • snRNP protein A antibody
    • SNRPA antibody
    • SNRPA_HUMAN antibody
    • U1 small nuclear ribonucleoprotein A antibody
    • U1 small nuclear RNP specific A antibody
    • U1 snRNP A antibody
    • U1 snRNP specific protein A antibody
    • U1-A antibody
    • U1A antibody
    • wu:fc19d01 antibody
    • zgc:77810 antibody
    see all

Images

  • Anti-U1A antibody (ab167613) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size: 31 kDa

  • All lanes : Anti-U1A antibody (ab167613) at 1 µg/ml

    Lane 1 : U1A transfected lysate 293T cell (31.02 KDa).
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 31 kDa

  • Immunofluorescent analysis of HeLa cells labeling U1A with ab167613 at 10 µg/ml.

References

ab167613 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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