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Rabbit monoclonal [EPR10632] to U1A
ICC/IF more details Unsuitable for:
Flow Cyt or IHC-P
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human U1A (UniProt ID: P09012).
HeLa, Jurkat, SH SY5Y and 293T cell lysates; 293T cells.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
1/10 - 1/100.
1/50 - 1/100.
Is unsuitable for Flow Cyt or IHC-P.
Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
Belongs to the RRM U1 A/B'' family.
Contains 2 RRM (RNA recognition motif) domains.
Information by UniProt
small nuclear ribonucleoprotein polypeptide A antibody
Western blot - Anti-U1A antibody [EPR10632] (ab166890)
All lanes : Anti-U1A antibody [EPR10632] (ab166890) at 1/10000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : SH SY5Y cell lysate Lane 4 : 293T cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 31 kDa
Immunoprecipitation - Anti-U1A antibody [EPR10632] (ab166890)
Western blot analysis (using ab166890 at 1/10000 dilution) of pellet from Jurkat cell lysate immunoprecipitated using ab166890 at 1/10000 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody [EPR10632] (ab166890)
Immunofluorescent analysis of 293T cells labeling U1A with ab166890 at a 1/50 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"