Overview

  • Product name
    Anti-U1A antibody [EPR10632]
    See all U1A primary antibodies
  • Description
    Rabbit monoclonal [EPR10632] to U1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human U1A (UniProt ID: P09012).

  • Positive control
    • HeLa, Jurkat, SH SY5Y and 293T cell lysates; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10632
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 31 kDa.
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
    • Sequence similarities
      Belongs to the RRM U1 A/B'' family.
      Contains 2 RRM (RNA recognition motif) domains.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • fc19d01 antibody
      • Mud1 antibody
      • small nuclear ribonucleoprotein polypeptide A antibody
      • snRNP A antibody
      • snRNP protein A antibody
      • SNRPA antibody
      • SNRPA_HUMAN antibody
      • U1 small nuclear ribonucleoprotein A antibody
      • U1 small nuclear RNP specific A antibody
      • U1 snRNP A antibody
      • U1 snRNP specific protein A antibody
      • U1-A antibody
      • U1A antibody
      • wu:fc19d01 antibody
      • zgc:77810 antibody
      see all

    Images

    • All lanes : Anti-U1A antibody [EPR10632] (ab166890) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SH SY5Y cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Western blot analysis (using ab166890 at 1/10000 dilution) of pellet from Jurkat cell lysate immunoprecipitated using ab166890 at 1/10000 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • Immunofluorescent analysis of 293T cells labeling U1A with ab166890 at a 1/50 dilution.

    References

    ab166890 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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