Product nameAnti-U1A antibody [EPR10632]
See all U1A primary antibodies
DescriptionRabbit monoclonal [EPR10632] to U1A
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human U1A (UniProt ID: P09012).
- HeLa, Jurkat, SH SY5Y and 293T cell lysates; 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 31 kDa.|
|IP||1/10 - 1/100.|
|ICC/IF||1/50 - 1/100.|
FunctionBinds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
Sequence similaritiesBelongs to the RRM U1 A/B'' family.
Contains 2 RRM (RNA recognition motif) domains.
- Information by UniProt
- fc19d01 antibody
- Mud1 antibody
- small nuclear ribonucleoprotein polypeptide A antibody
All lanes : Anti-U1A antibody [EPR10632] (ab166890) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Western blot analysis (using ab166890 at 1/10000 dilution) of pellet from Jurkat cell lysate immunoprecipitated using ab166890 at 1/10000 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of 293T cells labeling U1A with ab166890 at a 1/50 dilution.
ab166890 has not yet been referenced specifically in any publications.