Recombinant Anti-U2AF1L4 antibody [EPR14349] (ab188582)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14349] to U2AF1L4
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-U2AF1L4 antibody [EPR14349]
See all U2AF1L4 primary antibodies -
Description
Rabbit monoclonal [EPR14349] to U2AF1L4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, Human cerebellum, K562, Mouse brain and PC-12 lysates; U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14349 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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ICC/IF |
1/250.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
ICC/IF
1/250. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site. -
Tissue specificity
Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA splicing events. -
Cellular localization
Nucleus. Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 199746 Human
- Entrez Gene: 233073 Mouse
- Entrez Gene: 361542 Rat
- Omim: 601080 Human
- SwissProt: Q8WU68 Human
- SwissProt: Q8BGJ9 Mouse
- SwissProt: Q7TP17 Rat
- Unigene: 351558 Human
see all -
Alternative names
- FLJ35525 antibody
- MGC33901 antibody
- Splicing factor U2AF 26 kDa subunit antibody
see all
Images
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ab188582 staining U2AF1L4 in A549 (human lung carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Immunofluorescent analysis of paraformaldehyde-fixed U87-MG cells labeling U2AF1L4 with ab188582 at 1/250 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
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All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582)
Lane 1 : Mouse brain lysate
Lane 2 : PC-12 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-U2AF1L4 antibody [EPR14349] (ab188582) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : K562 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 26 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab188582 has not yet been referenced specifically in any publications.