Anti-U2AF35/U2AF1 antibody (ab86305)
Key features and details
- Rabbit polyclonal to U2AF35/U2AF1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-U2AF35/U2AF1 antibody
See all U2AF35/U2AF1 primary antibodies -
Description
Rabbit polyclonal to U2AF35/U2AF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human U2AF35/U2AF1 aa 140-190.
Database link: NP_006749.1 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab86305 was affinity purified using an epitope specific to U2AF35/U2AF1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 28 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 395370 Chicken
- Entrez Gene: 7307 Human
- Entrez Gene: 108121 Mouse
- Entrez Gene: 687575 Rat
- Entrez Gene: 733934 Xenopus tropicalis
- Entrez Gene: 192328 Zebrafish
- GenBank: BC001177 Human
- Omim: 191317 Human
see all -
Alternative names
- DKFZp313J1712 antibody
- FP793 antibody
- Human U2 snRNP auxiliary factor small subunit9 antibody
see all
Images
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All lanes : Anti-U2AF35/U2AF1 antibody (ab86305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling U2AF35/U2AF1 with ab86305 at 1/200 (1µg/ml). Detection: DAB.
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Detection of U2AF35/U2AF1 by Western Blot of Immunprecipitate.
ab86305, at 1 µg/ml, staining U2AF35/U2AF1 in HeLa whole cell lysate immunoprecipitated using ab86305 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab86305 has been referenced in 9 publications.
- Rivera OD et al. Alternative splicing redefines landscape of commonly mutated genes in acute myeloid leukemia. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33876749
- Chatrikhi R et al. A synthetic small molecule stalls pre-mRNA splicing by promoting an early-stage U2AF2-RNA complex. Cell Chem Biol 28:1145-1157.e6 (2021). PubMed: 33689684
- Yao J et al. Prevalent intron retention fine-tunes gene expression and contributes to cellular senescence. Aging Cell 19:e13276 (2020). PubMed: 33274830
- Chang JW et al. mTOR-regulated U2af1 tandem exon splicing specifies transcriptome features for translational control. Nucleic Acids Res 47:10373-10387 (2019). PubMed: 31504847
- Smith MA et al. U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies. Nat Cell Biol 21:640-650 (2019). PubMed: 31011167
- Královicová J et al. PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM. Nucleic Acids Res 46:6166-6187 (2018). PubMed: 29788428
- Martinez NM et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev 29:2054-66 (2015). PubMed: 26443849
- Zhang J et al. Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinities. Proc Natl Acad Sci U S A 112:E4726-34 (2015). WB . PubMed: 26261309
- Shao C et al. Mechanisms for U2AF to define 3' splice sites and regulate alternative splicing in the human genome. Nat Struct Mol Biol 21:997-1005 (2014). PubMed: 25326705