Product nameAnti-U2AF35/U2AF1 antibody
See all U2AF35/U2AF1 primary antibodies
DescriptionRabbit polyclonal to U2AF35/U2AF1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human U2AF35/U2AF1 aa 140-190.
Database link: NP_006749.1
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as U2AF35
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86305 was affinity purified using an epitope specific to U2AF35/U2AF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 28 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 2 C3H1-type zinc fingers.
Contains 1 RRM (RNA recognition motif) domain.
DomainThe C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Nucleus speckle.
- Information by UniProt
- DKFZp313J1712 antibody
- FP793 antibody
- Human U2 snRNP auxiliary factor small subunit9 antibody
All lanes : Anti-U2AF35/U2AF1 antibody (ab86305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling U2AF35/U2AF1 with ab86305 at 1/200 (1µg/ml). Detection: DAB.
Detection of U2AF35/U2AF1 by Western Blot of Immunprecipitate.
ab86305, at 1 µg/ml, staining U2AF35/U2AF1 in HeLa whole cell lysate immunoprecipitated using ab86305 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Královicová J et al. PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM. Nucleic Acids Res 46:6166-6187 (2018). Read more (PubMed: 29788428) »
- Martinez NM et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev 29:2054-66 (2015). Read more (PubMed: 26443849) »