Anti-U2AF35/U2AF1 antibody (ab86305)

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Western blot - Anti-U2AF35/U2AF1 antibody (ab86305)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody (ab86305)
  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (ab86305)

Key features and details

  • Rabbit polyclonal to U2AF35/U2AF1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-U2AF35/U2AF1 antibody
    See all U2AF35/U2AF1 primary antibodies

  • Description

    Rabbit polyclonal to U2AF35/U2AF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis

  • Immunogen

    Synthetic peptide corresponding to Human U2AF35/U2AF1 aa 140-190.
    Database link: NP_006749.1

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.

  • General notes

     This product was previously labelled as U2AF35

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab86305 was affinity purified using an epitope specific to U2AF35/U2AF1 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab86305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 2 C3H1-type zinc fingers.
    Contains 1 RRM (RNA recognition motif) domain.

  • Domain

    The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Nucleus speckle.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp313J1712 antibody
    • FP793 antibody
    • Human U2 snRNP auxiliary factor small subunit9 antibody
    • RN antibody
    • RNU2AF1 antibody
    • Splicing factor U2AF 35 kDa subunit antibody
    • U2 auxiliary factor 35 kDa subunit antibody
    • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
    • U2 small nuclear RNA auxiliary factor 1 antibody
    • U2 snRNP auxiliary factor small subunit antibody
    • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
    • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
    • U2AF1 antibody
    • U2AF1_HUMAN antibody
    • U2AF35 antibody
    • U2AFBP antibody
    see all

Images

  • Western blot - Anti-U2AF35/U2AF1 antibody (ab86305)
    Western blot - Anti-U2AF35/U2AF1 antibody (ab86305)
    All lanes : Anti-U2AF35/U2AF1 antibody (ab86305) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody (ab86305)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody (ab86305)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse CT26 colon carcinoma (right) tissues labelling U2AF35/U2AF1 with ab86305 at 1/200 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (ab86305)
    Immunoprecipitation - Anti-U2AF35/U2AF1 antibody (ab86305)

    Detection of U2AF35/U2AF1 by Western Blot of Immunprecipitate.
    ab86305, at 1 µg/ml, staining U2AF35/U2AF1 in HeLa whole cell lysate immunoprecipitated using ab86305 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References (5)

Publishing research using ab86305? Please let us know so that we can cite the reference in this datasheet.

ab86305 has been referenced in 5 publications.

  • Chang JW  et al. mTOR-regulated U2af1 tandem exon splicing specifies transcriptome features for translational control. Nucleic Acids Res 47:10373-10387 (2019). PubMed: 31504847
  • Královicová J  et al. PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM. Nucleic Acids Res 46:6166-6187 (2018). PubMed: 29788428
  • Martinez NM  et al. Widespread JNK-dependent alternative splicing induces a positive feedback loop through CELF2-mediated regulation of MKK7 during T-cell activation. Genes Dev 29:2054-66 (2015). PubMed: 26443849
  • Zhang J  et al. Disease-associated mutation in SRSF2 misregulates splicing by altering RNA-binding affinities. Proc Natl Acad Sci U S A 112:E4726-34 (2015). WB . PubMed: 26261309
  • Shao C  et al. Mechanisms for U2AF to define 3' splice sites and regulate alternative splicing in the human genome. Nat Struct Mol Biol 21:997-1005 (2014). PubMed: 25326705

Customer reviews and Q&As

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Western blot abreview for Anti-U2AF35 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Fibroblast)
Loading amount
20 µg
Specification
Human Fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 11 2013

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