For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    u2af35u2af1-antibody-epr12649b-ab172614.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
Share by email
RecombinantRabMAb

Recombinant Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

  • Datasheet
Reviews (1) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-RING1 antibody [EPR13047] (ab180170)

View more associated products

Overview

  • Product name

    Anti-U2AF35/U2AF1 antibody [EPR12649(B)]
    See all U2AF35/U2AF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human U2AF35/U2AF1 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01081

  • Positive control

    • HeLa, 293T, Ramos and NIH 3T3 cell lysates; HeLa cells; Human endometrial carcinoma and Human pancreas tissues.
  • General notes

     This product was previously labelled as U2AF35

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12649(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Alternative Versions

    • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] - BSA and Azide free (ab249709)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Ramos whole cell lysate (ab3955)
    • NIH 3T3 whole cell lysate (ab7179)
  • Related Products

    • Recombinant Human U2AF35/U2AF1 protein (denatured) (ab156324)

Applications

Our Abpromise guarantee covers the use of ab172614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
    • Sequence similarities

      Belongs to the splicing factor SR family.
      Contains 2 C3H1-type zinc fingers.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain

      The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus. Nucleus speckle.
    • Target information above from: UniProt accession Q01081 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7307 Human
      • Entrez Gene: 108121 Mouse
      • Entrez Gene: 687575 Rat
      • GenBank: BC001177 Human
      • Omim: 191317 Human
      • SwissProt: Q01081 Human
      • SwissProt: Q9D883 Mouse
      • Unigene: 365116 Human
      • Unigene: 379289 Mouse
      see all
    • Alternative names

      • DKFZp313J1712 antibody
      • FP793 antibody
      • Human U2 snRNP auxiliary factor small subunit9 antibody
      • RN antibody
      • RNU2AF1 antibody
      • Splicing factor U2AF 35 kDa subunit antibody
      • U2 auxiliary factor 35 kDa subunit antibody
      • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
      • U2 small nuclear RNA auxiliary factor 1 antibody
      • U2 snRNP auxiliary factor small subunit antibody
      • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
      • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
      • U2AF1 antibody
      • U2AF1_HUMAN antibody
      • U2AF35 antibody
      • U2AFBP antibody
      see all

    Images

    • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      All lanes : Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunofluorescent analysis of HeLa cells labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

    • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab172614 at 1/10 dilution.

    • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab172614? Please let us know so that we can cite the reference in this datasheet.

    ab172614 has been referenced in 2 publications.

    • Esfahani MS  et al. Functional significance of U2AF1 S34F mutations in lung adenocarcinomas. Nat Commun 10:5712 (2019). PubMed: 31836708
    • Palangat M  et al. The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation. Genes Dev 33:482-497 (2019). PubMed: 30842218

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK21-T7)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    BHK21-T7
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Simon Boudreault

    Verified customer

    Submitted Sep 16 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.