RecombinantRabMAb

Recombinant Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

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Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
  • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-U2AF35/U2AF1 antibody [EPR12649(B)]
    See all U2AF35/U2AF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12649(B)] to U2AF35/U2AF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human U2AF35/U2AF1 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q01081

  • Positive control

    • HeLa, 293T, Ramos and NIH 3T3 cell lysates; HeLa cells; Human endometrial carcinoma and Human pancreas tissues.

  • General notes

     This product was previously labelled as U2AF35

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12649(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

    • Sequence similarities

      Belongs to the splicing factor SR family.
      Contains 2 C3H1-type zinc fingers.
      Contains 1 RRM (RNA recognition motif) domain.

    • Domain

      The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus. Nucleus speckle.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • DKFZp313J1712 antibody
      • FP793 antibody
      • Human U2 snRNP auxiliary factor small subunit9 antibody
      • RN antibody
      • RNU2AF1 antibody
      • Splicing factor U2AF 35 kDa subunit antibody
      • U2 auxiliary factor 35 kDa subunit antibody
      • U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit antibody
      • U2 small nuclear RNA auxiliary factor 1 antibody
      • U2 snRNP auxiliary factor small subunit antibody
      • U2(RNU2) small nuclear RNA auxiliary factor 1 antibody
      • U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody
      • U2AF1 antibody
      • U2AF1_HUMAN antibody
      • U2AF35 antibody
      • U2AFBP antibody
      see all

    Images

    • Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Western blot - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      All lanes : Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Immunofluorescent analysis of HeLa cells labeling U2AF35/U2AF1 with ab172614 at 1/50 dilution.

    • Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Immunoprecipitation - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab172614 at 1/10 dilution.

    • Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)
      Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (ab172614)

    References (2)

    Publishing research using ab172614? Please let us know so that we can cite the reference in this datasheet.

    ab172614 has been referenced in 2 publications.

    • Esfahani MS  et al. Functional significance of U2AF1 S34F mutations in lung adenocarcinomas. Nat Commun 10:5712 (2019). PubMed: 31836708
    • Palangat M  et al. The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation. Genes Dev 33:482-497 (2019). PubMed: 30842218

    Customer reviews and Q&As

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    Western blot abreview for Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (BHK21-T7)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    BHK21-T7
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Simon Boudreault

    Verified customer

    Submitted Sep 16 2020

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