Overview

  • Product name
    U87 MG nuclear extract lysate
  • General notes
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    Glioblastoma is the highest dedifferentiated form of astrocytic brain tumours, and it is refractory to chemotherapy in most cases. U-87 MG (malignant glioma) cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 (Par-4) which is upregulated in prostate tumor cells undergoing apoptosis (several Abcam Par-4 abs). It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain.

References

ab14903 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for your inquiry.

Unfortunately, I am sorry to confirm we do not sell U-87 MG whole cell lysate, only the nuclear lysate (ab14903).

I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:

http://www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

I'm sorry we do not have the specific product for your requirements on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Answer

Thank you for your interest in ab14903. This lysate is a protein lysate only and is not suitable for DNA/RNA work. Unfortunately we do not have cell lines or tissues available for RNA isolation on our catalog. I apologize for any inconvenience this may cause. Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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