U937 cytoplasmic extract lysate (ab14904)

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Overview

  • Product name

    U937 cytoplasmic extract lysate
    See all U937 lysates

  • General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). 

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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  • Research areas

  • Background

    The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14904? Please let us know so that we can cite the reference in this datasheet.

ab14904 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

I would like to know if this cell line (U937) is an adherent type or not. I am cultivating these cells and would like to infect them with Leishmania parasite to determine their in vitro sensitivity to some antileishmanial drugs. If this cell line in not an adherent type, please advise on how could I make them to be adhered to the culture flasks or testing chamber slides.

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Answer

Thank you for your enquiry. U937 cells grow in suspension. To make them adhere, you can try using poly-lysine, fibronectin or collagen coated plates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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