Anti-UACA/Nucling antibody (ab233432)
Key features and details
- Rabbit polyclonal to UACA/Nucling
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-UACA/Nucling antibody
See all UACA/Nucling primary antibodies -
Description
Rabbit polyclonal to UACA/Nucling -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Dog -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human UACA/Nucling aa 1-250. N-terminal tags. (Expressed in E.coli).
Database link: Q9BZF9 -
Positive control
- WB: HeLa whole cell lysate (ab150035); Cow pancreas lysate; Recombinant human UACA/Nucling protein. IHC-P: Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 163 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 163 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury. -
Tissue specificity
Highly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells. -
Sequence similarities
Contains 6 ANK repeats. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281559 Cow
- Entrez Gene: 55075 Human
- Entrez Gene: 72565 Mouse
- Omim: 612516 Human
- SwissProt: Q8HYY4 Cow
- SwissProt: Q9BZF9 Human
- SwissProt: Q8CGB3 Mouse
- Unigene: 108049 Human
see all -
Alternative names
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human brain tissue stained for UACA/Nucling using ab233432 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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All lanes : Anti-UACA/Nucling antibody (ab233432) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Cow pancreas tissue
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 163 kDa -
Anti-UACA/Nucling antibody (ab233432) at 2 µg/ml + Recombinant human UACA/Nucling protein
Predicted band size: 163 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233432 has not yet been referenced specifically in any publications.