Key features and details
- Rabbit polyclonal to UACA/Nucling
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
Product nameAnti-UACA/Nucling antibody
See all UACA/Nucling primary antibodies
DescriptionRabbit polyclonal to UACA/Nucling
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Cow, Human
Predicted to work with: Mouse, Dog
Recombinant fragment (His-T7-tag) corresponding to Human UACA/Nucling aa 29-209. N-terminal tags. (Expressed in E.coli).
ADWNKYDDRLMKAAERGDVEKVTSILAKKGVNPGKLDVEGRSVFHVVTSK GNLECLNAILIHGVDITTSDTAGRNALHLAAKYGHALCLQKLLQYNCPTE HADLQGRTALHDAAMADCPSSIQLLCDHGASVNAKDVDGRTPLVLATQMS RPTICQLLIDRGADVNSRDKQNRTALMLGCE
Database link: Q9BZF9
- WB: HeLa whole cell lysate (ab150035); Cow pancreas lysate; Recombinant human UACA/Nucling protein. IHC-P: Human brain tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 163 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
Sequence similaritiesContains 6 ANK repeats.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
- Information by UniProt
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for UACA/Nucling using ab233432 at 20 µg/mL in immunohistochemical analysis. DAB staining.
All lanes : Anti-UACA/Nucling antibody (ab233432) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Cow pancreas tissue
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 163 kDa
Anti-UACA/Nucling antibody (ab233432) at 2 µg/ml + Recombinant human UACA/Nucling protein
Predicted band size: 163 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233432 has not yet been referenced specifically in any publications.