Key features and details
- Rabbit polyclonal to UACA/Nucling
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-UACA/Nucling antibody
See all UACA/Nucling primary antibodies
DescriptionRabbit polyclonal to UACA/Nucling
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 162 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRegulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury.
Tissue specificityHighly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells.
Sequence similaritiesContains 6 ANK repeats.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm.
- Information by UniProt
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
All lanes : Anti-UACA/Nucling antibody (ab99323) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 162 kDa
Exposure time: 10 seconds
Detection of UACA/Nucling in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded), using ab99323 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab99323 has not yet been referenced specifically in any publications.