Anti-UACA/Nucling antibody [UACA/1222] (ab233956)
![Western blot - Anti-UACA/Nucling antibody [UACA/1222] (ab233956)](https://www.abcam.com/ps/products/233/ab233956/Images/ab233956-308039-ab233956.jpg "Western blot - Anti-UACA/Nucling antibody [UACA/1222] (ab233956)")
Key features and details
- Mouse monoclonal [UACA/1222] to UACA/Nucling
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-UACA/Nucling antibody [UACA/1222]
See all UACA/Nucling primary antibodies -
Description
Mouse monoclonal [UACA/1222] to UACA/Nucling -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human UACA/Nucling.
Database link: Q9BZF9 -
Positive control
- WB: A549 cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
UACA/1222 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab233956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 163 kDa. |
Target
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Function
Regulates APAF1 expression and plays an important role in the regulation of stress-induced apoptosis. Promotes apoptosis by regulating three pathways, apoptosome up-regulation, LGALS3/galectin-3 down-regulation and NF-kappa-B inactivation. Regulates the redistribution of APAF1 into the nucleus after proapoptotic stress. Down-regulates the expression of LGALS3 by inhibiting NFKB1.
Modulates isoactin dynamics to regulate the morphological alterations required for cell growth and motility. Interaction with ARF6 may modulate cell shape and motility after injury. -
Tissue specificity
Highly expressed in skeletal muscle, heart, kidney and pancreas. Expressed in choroid, retina and epidermal melanocytes. Expressed in eye muscles and thyroid follicular cells. -
Sequence similarities
Contains 6 ANK repeats. -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Expressed diffusely in cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55075 Human
- Omim: 612516 Human
- SwissProt: Q9BZF9 Human
- Unigene: 108049 Human
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Alternative names
- FLJ10128 antibody
- KIAA1561 antibody
- MGC141967 antibody
see all
Images
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Western blot - Anti-UACA/Nucling antibody [UACA/1222] (ab233956)Anti-UACA/Nucling antibody [UACA/1222] (ab233956) at 1 µg/ml + A549 (Human lung carcinoma cell line) cell lysate
Predicted band size: 163 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab233956 has not yet been referenced specifically in any publications.
