Recombinant
RabMAb

Recombinant Anti-UAP1 antibody [EPR10259] (Phycoerythrin) (ab213187)

Overview

  • Product name

    Anti-UAP1 antibody [EPR10259] (Phycoerythrin)
    See all UAP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10259] to UAP1 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UAP1 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16222

  • Positive control

    • Flow Cytometry: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/5000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.
  • Tissue specificity

    Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver.
  • Pathway

    Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.
  • Sequence similarities

    Belongs to the UDPGP type 1 family.
  • Cellular localization

    Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGX 1 antibody
    • AGX antibody
    • AGX-1 antibody
    • AGX-2 antibody
    • AGX1 antibody
    • Antigen X antibody
    • AntigenX antibody
    • SPAG 2 antibody
    • SPAG2 antibody
    • Sperm associated antigen 2 antibody
    • Sperm-associated antigen 2 antibody
    • UAP 1 antibody
    • uap1 antibody
    • UAP1_HUMAN antibody
    • UDP N acetylhexosamine pyrophosphorylase antibody
    • UDP N acteylglucosamine pyrophosphorylase 1 antibody
    • UDP-N-acetylglucosamine pyrophosphorylase antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab213187 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213187, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

References

ab213187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213187.
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