Recombinant
RabMAb

Recombinant Anti-UAP1L1 antibody [EPR11586] (ab174855)

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Overview

  • Product name

    Anti-UAP1L1 antibody [EPR11586]
    See all UAP1L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11586] to UAP1L1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UAP1L1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q3KQV9

  • Positive control

    • Caco 2 and K562 cell lysates; K562 cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11586

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    Images

    • Flow Cytometry - Anti-UAP1L1 antibody [EPR11586] (ab174855)
      Flow Cytometry - Anti-UAP1L1 antibody [EPR11586] (ab174855)

      Flow cytometric analysis of permeabilized K562 cells labeling UAP1L1 using ab174855 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence - Anti-UAP1L1 antibody [EPR11586] (ab174855)
      Immunocytochemistry/ Immunofluorescence - Anti-UAP1L1 antibody [EPR11586] (ab174855)

      Immunofluorescent staining of K562 cells labeling UAP1L1 using ab174855 at 1/50 dilution (red). DAPI nuclear staining (blue).

    • Western blot - Anti-UAP1L1 antibody [EPR11586] (ab174855)
      Western blot - Anti-UAP1L1 antibody [EPR11586] (ab174855)
      All lanes : Anti-UAP1L1 antibody [EPR11586] (ab174855) at 1/1000 dilution

      Lane 1 : Caco 2 cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    References

    ab174855 has not yet been referenced specifically in any publications.

    Publishing research using ab174855? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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