Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityProtein G purified
Purification notesFiltered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab50986 in the following tested applications.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 50 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
Sequence similaritiesBelongs to the DEAD box helicase family. DECD subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe helicase C-terminal domain mediates interaction with THOC4.
Cellular localizationNucleus. Nucleus speckle.
- Information by UniProt
- 0610030D10Rik antibody
- 4F2-LC6 antibody
- 56 kDa U2AF65-associated protein antibody
Anti-UAP56 antibody [2060C10a] (ab50986) + immunizing peptide
Predicted band size: 50 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab50986 has not yet been referenced specifically in any publications.