Key features and details
- Rabbit monoclonal [EPR13144-62] to UAP56
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-UAP56 antibody [EPR13144-62]
See all UAP56 primary antibodies
DescriptionRabbit monoclonal [EPR13144-62] to UAP56
Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human UAP56 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q13838
- K562, HeLa, Jurkat and 293 cell lysate. Human infiltrating duct breast carcinoma tissue. HeLa and Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181061 in the following tested applications.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionComponent of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
Sequence similaritiesBelongs to the DEAD box helicase family. DECD subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
DomainThe helicase C-terminal domain mediates interaction with THOC4.
Cellular localizationNucleus. Nucleus speckle.
- Information by UniProt
- 0610030D10Rik antibody
- 4F2-LC6 antibody
- 56 kDa U2AF65-associated protein antibody
All lanes : Anti-UAP56 antibody [EPR13144-62] (ab181061) at 1/20000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa
Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunohistochemical analysis of paraffin embedded Human infiltrating duct breast carcinoma tissue sections labeling UAP56 using ab181061 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181061 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.
Flow Cytometry analysis of Jurkat cells labeling UAP56 using ab181061 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
ab181061 has been referenced in 2 publications.
- Awasthi S et al. DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA. Nucleic Acids Res 46:6304-6317 (2018). PubMed: 29618122
- Awasthi S et al. DDX39B promotes translation through regulation of pre-ribosomal RNA levels. RNA Biol 15:1157-1166 (2018). PubMed: 30176153