Anti-UAP56 antibody [EPR13144-62] (ab181061)


  • Product name
    Anti-UAP56 antibody [EPR13144-62]
    See all UAP56 primary antibodies
  • Description
    Rabbit monoclonal [EPR13144-62] to UAP56
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human UAP56 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13838

  • Positive control
    • K562, HeLa, Jurkat and 293 cell lysate. Human infiltrating duct breast carcinoma tissue. HeLa and Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab181061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.


  • Function
    Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
    Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DECD subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The helicase C-terminal domain mediates interaction with THOC4.
  • Cellular localization
    Nucleus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610030D10Rik antibody
    • 4F2-LC6 antibody
    • 56 kDa U2AF65-associated protein antibody
    • AI428441 antibody
    • ATP-dependent RNA helicase p47 antibody
    • B(0,+)-type amino acid transporter 1 antibody
    • BAT1 antibody
    • Bat1a antibody
    • D17H6S81E antibody
    • D17H6S81E-1 antibody
    • D6S81E antibody
    • D6S81Eh antibody
    • DDX39B antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody
    • DEAD box protein UAP56 antibody
    • DX39B_HUMAN antibody
    • EC 3.6.1.- antibody
    • Glycoprotein-associated amino acid transporter b0,+AT1 antibody
    • HLA-B-associated transcript 1 protein antibody
    • HLA-B-associated transcript 1A antibody
    • HLA-B-associated transcript-1 antibody
    • MGC127051 antibody
    • MGC19235 antibody
    • MGC38799 antibody
    • nuclear RNA helicase (DEAD family) antibody
    • OTTHUMP00000029229 antibody
    • OTTHUMP00000165889 antibody
    • OTTHUMP00000165890 antibody
    • p47 antibody
    • Solute carrier family 7 member 9 antibody
    • Spliceosome RNA helicase BAT1 antibody
    • Spliceosome RNA helicase DDX39B antibody
    • U2AF65-associayed protein, 56-KD antibody
    • UAP56 antibody
    see all


  • All lanes : Anti-UAP56 antibody [EPR13144-62] (ab181061) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa

    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin embedded Human infiltrating duct breast carcinoma tissue sections labeling UAP56 using ab181061 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • Immunofluorescent analysis of acetone fixed HeLa cells labeling UAP56 using ab181061 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry analysis of Jurkat cells labeling UAP56 using ab181061 at a 1/10 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.


ab181061 has not yet been referenced specifically in any publications.

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