Overview

  • Product name

    Anti-UBA2 antibody - C-terminal
  • Description

    Rabbit polyclonal to UBA2 - C-terminal
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total UBA2 protein.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human UBA2 aa 591-640 (C terminal). The exact sequence is proprietary. NP_005490.1.
    Sequence:

    DSDEEDSSNN ADVSEEERSR KRKLDEKENL SAKRSRIEQK EELDDVIALD


    Database link: Q9UBT2

  • Positive control

    • Human testis tissue; UV treated 293 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab189289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 71 kDa.

Target

  • Relevance

    Function: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Pathway: Protein modification; protein sumoylation. Similarity: Belongs to the ubiquitin-activating E1 family. PTM: Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.
  • Database links

  • Alternative names

    • Anthracycline-associated resistance ARX antibody
    • SAE2_HUMAN antibody
    • SUMO-activating enzyme subunit 2 antibody
    • UBA2 antibody
    • Ubiquitin-like 1-activating enzyme E1B antibody
    • Ubiquitin-like modifier-activating enzyme 2 antibody
    see all

Images

  • All lanes : Anti-UBA2 antibody - C-terminal (ab189289) at 1/500 dilution

    Lane 1 : 293 cell extracts, treated with UV 5'
    Lane 2 : 293 cell extracts, treated with UV 5' with synthesized peptide

    Predicted band size: 71 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling UBA2 with ab189289 at 10µg/ml.

References

ab189289 has not yet been referenced specifically in any publications.

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