Key features and details
- Rabbit polyclonal to UBA2 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBA2 antibody - C-terminal
DescriptionRabbit polyclonal to UBA2 - C-terminal
SpecificityThis antibody detects endogenous levels of total UBA2 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Human testis tissue; UV treated 293 cell extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 71 kDa.|
RelevanceFunction: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Pathway: Protein modification; protein sumoylation. Similarity: Belongs to the ubiquitin-activating E1 family. PTM: Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.
- Anthracycline-associated resistance ARX antibody
- SAE2_HUMAN antibody
- SUMO-activating enzyme subunit 2 antibody
All lanes : Anti-UBA2 antibody - C-terminal (ab189289) at 1/500 dilution
Lane 1 : 293 cell extracts, treated with UV 5'
Lane 2 : 293 cell extracts, treated with UV 5' with synthesized peptide
Predicted band size: 71 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling UBA2 with ab189289 at 10µg/ml.
ab189289 has not yet been referenced specifically in any publications.