Anti-UBA3 antibody (ab247153)
Key features and details
- Rabbit polyclonal to UBA3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBA3 antibody
See all UBA3 primary antibodies -
Description
Rabbit polyclonal to UBA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human UBA3 aa 141-226.
Sequence:VVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVL DPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLE
Database link: Q8TBC4 -
Positive control
- IHC-P: Human skin tissue. ICC/IF: SiHa cells. WB: RT4 and U-251 MG whole cell lysate.
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General notes
This product was previously labelled as UBE1C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab247153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.04 - 0.4 µg/ml. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Protein modification; protein neddylation. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. - Information by UniProt
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Database links
- Entrez Gene: 9039 Human
- Omim: 603172 Human
- SwissProt: Q8TBC4 Human
- Unigene: 154320 Human
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Alternative names
- DKFZp566J164 antibody
- EC 6.3.2. antibody
- hUba3 antibody
see all
Images
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Western blot - Anti-UBA3 antibody (ab247153)All lanes : Anti-UBA3 antibody (ab247153) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate -
Immunocytochemistry/ Immunofluorescence - Anti-UBA3 antibody (ab247153)PFA-fixed, Triton X-100 permeabilized SiHA cells stained for UBA3 (green) using ab247153 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA3 antibody (ab247153)Paraffin-embedded human pancreas tissue stained for UBA3 using ab247153 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA3 antibody (ab247153)Paraffin-embedded human skin tissue stained for UBA3 using ab247153 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab247153 has not yet been referenced specifically in any publications.
