Overview

  • Product name

    Anti-UBA3 antibody [MILR42872]
    See all UBA3 primary antibodies
  • Description

    Rabbit monoclonal [MILR42872] to UBA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UBA3. The exact sequence is proprietary.

  • Positive control

    • Lysates of: fetal lung, 293T, HeLa, RAW 264.7, HepG2, K562
  • General notes

     

     

     This product was previously labelled as UBE1C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    MILR42872
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
    • Tissue specificity

      Ubiquitously expressed.
    • Pathway

      Protein modification; protein neddylation.
    • Sequence similarities

      Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp566J164 antibody
      • EC 6.3.2. antibody
      • hUba3 antibody
      • MGC22384 antibody
      • NEDD8 activating enzyme E1 catalytic subunit antibody
      • NEDD8 activating enzyme E1C antibody
      • Nedd8 activating enzyme hUba3 antibody
      • NEDD8-activating enzyme E1 catalytic subunit antibody
      • NEDD8-activating enzyme E1C antibody
      • uba3 antibody
      • UBA3 ubiquitin activating enzyme E1 homolog antibody
      • UBA3_HUMAN antibody
      • UBE1C antibody
      • Ubiquitin activating enzyme 3 antibody
      • Ubiquitin activating enzyme E1C antibody
      • Ubiquitin-activating enzyme 3 antibody
      • Ubiquitin-activating enzyme E1C antibody
      • Ubiquitin-like modifier-activating enzyme 3 antibody
      see all

    Images

    • All lanes : Anti-UBA3 antibody [MILR42872] (ab124728)

      Lane 1 : Fetal lung cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 49 kDa

    References

    This product has been referenced in:

    • Zhou Q  et al. Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells. JCI Insight 4:N/A (2019). Read more (PubMed: 30668548) »
    • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124728.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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