Key features and details
- Rabbit polyclonal to UBA5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBA5 antibody
See all UBA5 primary antibodies
DescriptionRabbit polyclonal to UBA5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Gorilla
- Jurkat, 293T and HeLa whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab177507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 45 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionE1-like enzyme which activates UFM1 and SUMO2.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family. UBA5 subfamily.
Cellular localizationCytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
- Information by UniProt
- 5730525G14Rik antibody
- AW240750 antibody
- FLJ23251 antibody
All lanes : Anti-UBA5 antibody (ab177507) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 seconds
Detection of UBA5 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177507 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab177507 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab177507 has not yet been referenced specifically in any publications.