Overview

  • Product name

    Anti-UBA5 antibody [EPR11729]
    See all UBA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR11729] to UBA5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBA5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9GZZ9

  • Positive control

    • Caco-2, Jurkat and 293T cell lysate: Human prostate gastric carcinoma and Human prostate tissues; Caco-2 cells; Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11729
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      E1-like enzyme which activates UFM1 and SUMO2.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.
    • Cellular localization

      Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5730525G14Rik antibody
      • AW240750 antibody
      • FLJ23251 antibody
      • FLJ23251UBA5 antibody
      • MGC105565 antibody
      • ThiFP1 antibody
      • UBA5 antibody
      • UBA5 ubiquitin activating enzyme E1 homolog antibody
      • UBA5_HUMAN antibody
      • UBE1DC antibody
      • UBE1DC1 antibody
      • Ubiquitin activating enzyme 5 antibody
      • Ubiquitin activating enzyme E1 domain containing 1 antibody
      • Ubiquitin activating enzyme E1 domain containing protein 1 antibody
      • Ubiquitin like modifier activating enzyme 5 antibody
      • Ubiquitin-activating enzyme 5 antibody
      • Ubiquitin-activating enzyme E1 domain-containing protein 1 antibody
      • Ubiquitin-like modifier-activating enzyme 5 antibody
      • UFM1 activating enzyme antibody
      • UFM1-activating enzyme antibody
      see all

    Images

    • All lanes : Anti-UBA5 antibody [EPR11729] (ab177478) at 1/1000 dilution

      Lane 1 : Caco-2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunofluorescent analysis of Caco-2 cells labeling UBA5 with ab177478 at 1/50 (green) and DAPI staining (blue).

    • Immunohistochemical analysis of paraffin embedded Human prostate gastric carcinoma tissue labeling UBA5 with ab177478 at 1/50.

    • Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling UBA5 with ab177478 at 1/50.

    • Flow Cytometrical analysis of permeabilized Jurkat cells labeling UBA5 with ab177478 at 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Retina Pigment Epithelium)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris (MOPS Buffer))
    Loading amount
    50 µg
    Specification
    Retina Pigment Epithelium
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jun 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up