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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBA5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9GZZ9
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab177478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunofluorescent analysis of Caco-2 cells labeling UBA5 with ab177478 at 1/50 (green) and DAPI staining (blue).
Immunohistochemical analysis of paraffin embedded Human prostate gastric carcinoma tissue labeling UBA5 with ab177478 at 1/50.
Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling UBA5 with ab177478 at 1/50.
Flow Cytometrical analysis of permeabilized Jurkat cells labeling UBA5 with ab177478 at 1/10 (red) or a rabbit IgG (negative) (green).
ab177478 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"