Recombinant Anti-UBA5 antibody [EPR11729] (ab177478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11729] to UBA5
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UBA5 antibody [EPR11729]
See all UBA5 primary antibodies -
Description
Rabbit monoclonal [EPR11729] to UBA5 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Caco-2, Jurkat and 293T cell lysate: Human prostate gastric carcinoma and Human prostate tissues; Caco-2 cells; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11729 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
1/50 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
E1-like enzyme which activates UFM1 and SUMO2. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. -
Cellular localization
Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2. - Information by UniProt
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Database links
- Entrez Gene: 79876 Human
- Omim: 610552 Human
- SwissProt: Q9GZZ9 Human
- Unigene: 170737 Human
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Alternative names
- 5730525G14Rik antibody
- AW240750 antibody
- FLJ23251 antibody
see all
Images
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All lanes : Anti-UBA5 antibody [EPR11729] (ab177478) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
Immunofluorescent analysis of Caco-2 cells labeling UBA5 with ab177478 at 1/50 (green) and DAPI staining (blue).
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Immunohistochemical analysis of paraffin embedded Human prostate gastric carcinoma tissue labeling UBA5 with ab177478 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling UBA5 with ab177478 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling UBA5 with ab177478 at 1/10 (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab177478 has been referenced in 2 publications.
- Qin B et al. STK38 promotes ATM activation by acting as a reader of histone H4 ufmylation. Sci Adv 6:eaax8214 (2020). PubMed: 32537488
- Qin B et al. UFL1 promotes histone H4 ufmylation and ATM activation. Nat Commun 10:1242 (2019). PubMed: 30886146