Overview

  • Product name

    Anti-UBA52 antibody [EPR4546] (HRP)
    See all UBA52 primary antibodies
  • Description

    Rabbit monoclonal [EPR4546] to UBA52 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBA52 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P62987

  • Positive control

    • WB: HEK293 and JAR whole cell lysate. IHC-P: normal human pancreas tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 8 kDa (predicted molecular weight: 15 kDa).

Target

  • Relevance

    Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
  • Cellular localization

    Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
  • Database links

  • Alternative names

    • 60S ribosomal protein L40 antibody
    • CEP52 antibody
    • HUBCEP52 antibody
    • L40 antibody
    • MGC126879 antibody
    • MGC57125 antibody
    • RPL40 antibody
    • UBA 52 antibody
    • Ubiquitin 52 amino acid fusion protein antibody
    • Ubiquitin 60S ribosomal protein L40 antibody
    • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
    • Ubiquitin carboxyl extension protein 52 antibody
    • Ubiquitin CEP52 antibody
    see all

Images

  • IHC image of UBA52 staining in a section of formalin-fixed paraffin-embedded normal human pancreas*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab208736, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-UBA52 antibody [EPR4546] (HRP) (ab208736) at 1/5000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : JAR (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208736 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208736 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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