Product nameAnti-UBA52 antibody [EPR4547]
See all UBA52 primary antibodies
DescriptionRabbit monoclonal [EPR4547] to UBA52
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human UBA52 aa 100-200. The exact sequence is proprietary.
- WB: Human placenta, Raji, fetal kidney, 293T and Jurkat lysates IHC-P: Human brain tissue IF/ICC: HepG2 cell line.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 15 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.|
|ICC/IF||1/250 - 1/500.|
RelevanceUbiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
Cellular localizationUbiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
- 60S ribosomal protein L40 antibody
- CEP52 antibody
- HUBCEP52 antibody
ab109230 stained HepG2 cells. The cells were 4% formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Raji cell lysate
Lane 3 : Fetal kidney lysate
Lane 4 : 293T cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue, using ab109230 at a 1/500 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.