Overview

  • Product name

  • Description

    Rabbit polyclonal to Uba6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Uba6 aa 1-44.
    Database link: A0AVT1

  • Positive control

    • IHC-P: Human brain and testicular tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab234833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.

Target

  • Function

    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
  • Tissue specificity

    Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • E1-L2 antibody
    • FLJ10808 antibody
    • FLJ23367 antibody
    • Monocyte protein 4 antibody
    • MOP 4 antibody
    • MOP-4 antibody
    • MOP4 antibody
    • UBA 6 antibody
    • Uba6 antibody
    • UBA6 ubiquitin activating enzyme E1 antibody
    • UBA6_HUMAN antibody
    • UBE1L2 antibody
    • Ubiquitin activating enzyme 6 antibody
    • Ubiquitin activating enzyme E1 like 2 antibody
    • Ubiquitin activating enzyme E1 like protein 2 antibody
    • Ubiquitin like modifier activating enzyme 6 antibody
    • Ubiquitin-activating enzyme 6 antibody
    • Ubiquitin-activating enzyme E1-like protein 2 antibody
    • Ubiquitin-like modifier-activating enzyme 6 antibody
    see all

Images

  • HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Uba6 (green) using ab234833 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human testicular tissue stained for Uba6 with ab234833 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human brain tissue stained for Uba6 with ab234833 at 1/100 dilution in immunohistochemical analysis.

References

ab234833 has not yet been referenced specifically in any publications.

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