Overview

  • Product name

  • Description

    Rabbit polyclonal to Uba6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Uba6 aa 1-50. The exact sequence is proprietary. (NP_060697.4 )
    Database link: A0AVT1

  • Positive control

    • WB: HEK-293T, HeLa and Jurkat whole cell lysates. IP: Uba6 IP HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab240702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 118 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
  • Tissue specificity

    Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • E1-L2 antibody
    • FLJ10808 antibody
    • FLJ23367 antibody
    • Monocyte protein 4 antibody
    • MOP 4 antibody
    • MOP-4 antibody
    • MOP4 antibody
    • UBA 6 antibody
    • Uba6 antibody
    • UBA6 ubiquitin activating enzyme E1 antibody
    • UBA6_HUMAN antibody
    • UBE1L2 antibody
    • Ubiquitin activating enzyme 6 antibody
    • Ubiquitin activating enzyme E1 like 2 antibody
    • Ubiquitin activating enzyme E1 like protein 2 antibody
    • Ubiquitin like modifier activating enzyme 6 antibody
    • Ubiquitin-activating enzyme 6 antibody
    • Ubiquitin-activating enzyme E1-like protein 2 antibody
    • Ubiquitin-like modifier-activating enzyme 6 antibody
    see all

Images

  • All lanes : Anti-Uba6 antibody (ab240702) at 0.1 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 118 kDa


    Exposure time: 3 minutes
  • Uba6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240702 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab240702 at 0.1 µg/ml.

    Lane 1: ab240702 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab240702 has not yet been referenced specifically in any publications.

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