Product nameAnti-Uba6 antibody
See all Uba6 primary antibodies
DescriptionRabbit polyclonal to Uba6
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Uba6 aa 1-50. The exact sequence is proprietary. (NP_060697.4 )
Database link: A0AVT1
- WB: HEK-293T, HeLa and Jurkat whole cell lysates. IP: Uba6 IP HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab240702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 118 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActivates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
Tissue specificityWidely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
- E1-L2 antibody
- FLJ10808 antibody
- FLJ23367 antibody
All lanes : Anti-Uba6 antibody (ab240702) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 3 minutes
Uba6 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240702 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab240702 at 0.1 µg/ml.
Lane 1: ab240702 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab240702 has not yet been referenced specifically in any publications.