Product nameAnti-UBAP2 antibody
See all UBAP2 primary antibodies
DescriptionRabbit polyclonal to UBAP2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Rhesus monkey, Gorilla
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab197083 was affinity purified using an epitope specific to UBAP2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab197083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 117 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesContains 1 UBA domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- AD-012 protein antibody
- bA176F3.5 antibody
- UBAP-2 antibody
All lanes : Anti-UBAP2 antibody (ab197083) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 117 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Detection of UBAP2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197083 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab197083 has not yet been referenced specifically in any publications.