Key features and details
- Rabbit polyclonal to UBAP2L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBAP2L antibody
See all UBAP2L primary antibodies
DescriptionRabbit polyclonal to UBAP2L
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide derived from internal sequence of Human UBAP2L.
- Jurkat whole cell lysate (ab7899)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 115 kDa.|
Sequence similaritiesContains 1 UBA domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- FLJ42300 antibody
- KIAA0144 antibody
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All lanes : Anti-UBAP2L antibody (ab138309) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate at 18 µg
Lane 2 : HeLa whole cell lysate at 36 µg
Lane 3 : RPE-1 whole cell lysate at 18 µg
Lane 4 : RPE-1 whole cell lysate at 36 µg
All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 115 kDa
Observed band size: 117,120 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa (possible non-specific binding)
Exposure time: 7 minutes
Blocking: 10% Milk
Anti-UBAP2L antibody (ab138309) at 1/500 dilution + Jurkat cell lysate at 30 µg
Predicted band size: 115 kDa
ab138309 has been referenced in 1 publication.
- Lund PJ et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). WB ; Human . PubMed: 27655845