Overview

  • Product name

  • Description

    Rabbit polyclonal to UBAP2L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal sequence of Human UBAP2L.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 115 kDa.

Target

Images

  • All lanes : Anti-UBAP2L antibody (ab138309) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate at 18 µg
    Lane 2 : HeLa whole cell lysate at 36 µg
    Lane 3 : RPE-1 whole cell lysate at 18 µg
    Lane 4 : RPE-1 whole cell lysate at 36 µg

    Secondary
    All lanes : LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 115 kDa
    Observed band size: 117,120 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 17 kDa (possible non-specific binding)


    Exposure time: 7 minutes


    Blocking: 10% Milk

    See Abreview

  • Anti-UBAP2L antibody (ab138309) at 1/500 dilution + Jurkat cell lysate at 30 µg

    Predicted band size: 115 kDa

References

This product has been referenced in:

  • Lund PJ  et al. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098 (2016). WB ; Human . Read more (PubMed: 27655845) »
See 1 Publication for this product

Customer reviews and Q&As

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (HeLa, RPE-1)
Specification
HeLa, RPE-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 01 2013

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