Overview

  • Product name

  • Description

    Rabbit polyclonal to UBAP2L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 400 and 450 of human UBAP2L (AAH03170.1).

  • Positive control

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at a concentration of 2 - 5 µg/mg.
    WB: 1/5000 - 1/25000. Detects a band of approximately 160 kDa (predicted molecular weight: 115 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 UBA domain.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Acetylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ42300 antibody
      • KIAA0144 antibody
      • NICE 4 antibody
      • NICE4 antibody
      • Protein NICE-4 antibody
      • Protein NICE4 antibody
      • Ubap2l antibody
      • Ubiquitin associated protein 2 like antibody
      • Ubiquitin-associated protein 2-like antibody
      • UBP2L_HUMAN antibody
      see all

    Images

    • All lanes : Anti-UBAP2L antibody (ab70319) at 0.03 µg/ml

      Lane 1 : Mock treated Hela cell lysate
      Lane 2 : Hela cell lysate treated with a control siRNA
      Lane 3 : Hela cell lysate treated with siRNA for UBAP2L

      Lysates/proteins at 100 µg per lane.

      Predicted band size: 115 kDa
      Observed band size: 160 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.



      Detection: Chemiluminescence with an exposure time of 15 seconds.

    References

    This product has been referenced in:

    • He J  et al. UBAP2L silencing inhibits cell proliferation and G2/M phase transition in breast cancer. Breast Cancer 25:224-232 (2018). WB ; Human . Read more (PubMed: 29196913) »
    • Lin GL  et al. Upregulation of UBAP2L in Bone Marrow Mesenchymal Stem Cells Promotes Functional Recovery in Rats with Spinal Cord Injury. Curr Med Sci 38:1081-1089 (2018). Read more (PubMed: 30536073) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70319.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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