• Product name
  • Description
    Goat polyclonal to UBC3B
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Electron Microscopymore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide: AQQQMTSSQKALM, corresponding to N terminal amino acids 2-14 of Human UBC3B.

  • General notes
    Principal Names – UBE2R2; UBC3B; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B. Official Gene Symbol - UBE2R2. GenBank Accession Number – NP_060281. LocusLink ID - 54926 (human); 67615 (mouse). Gene Ontology terms - ubiquitin conjugating enzyme activity; ubiquitin-protein ligase activity; ubiquitin cycle; protein modification; ligase activity. An Ubiquitin-conjugating enzyme.



Our Abpromise guarantee covers the use of ab4281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Can be blocked with Human UBC3B peptide (ab23017). No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml.
ELISA Use at an assay dependent concentration. antibody detection limit dilution 1/4000.
Electron Microscopy Use at an assay dependent concentration. This product stained chromatoid bodies in Rat spermatids.


  • Function
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-48'-linked polyubiquitination. May be involved in degradation of katenin.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDC34B antibody
    • E2 CDC34B antibody
    • E2 ubiquitin conjugating enzyme R2 antibody
    • FLJ20419 antibody
    • MGC10481 antibody
    • UB2R2 antibody
    • UB2R2_HUMAN antibody
    • UBC3B antibody
    • UBE2R2 antibody
    • Ubiquitin carrier protein R2 antibody
    • Ubiquitin conjugating enzyme E2 CDC34B antibody
    • Ubiquitin conjugating enzyme E2 R2 antibody
    • Ubiquitin conjugating enzyme E2R 2 antibody
    • Ubiquitin conjugating enzyme UBC3B antibody
    • Ubiquitin protein ligase R2 antibody
    • Ubiquitin-conjugating enzyme E2 R2 antibody
    • Ubiquitin-conjugating enzyme E2-CDC34B antibody
    • Ubiquitin-protein ligase R2 antibody
    see all


  • ab4281 (2µg/ml) shows staining of paraffin embedded Human Testis stains spermatocytes. Steamed antigen retrieval with citrate buffer pH 6 carried out and HRP-staining.


This product has been referenced in:
  • Haraguchi CM  et al. Chromatoid bodies: aggresome-like characteristics and degradation sites for organelles of spermiogenic cells. J Histochem Cytochem 53:455-65 (2005). Electron Microscopy ; Rat . Read more (PubMed: 15805420) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for your enquiry. As you are probably aware this product is a Fast-Track antibody and it is not fully characterized. Please take a look a the datasheet and read the Terms and Conditions for Fast Track antibodies We have not tested it for Western blot analysis in our lab only for ELISA. We had a quick look and can only find the predicted (by automated genome analysis) Rat sequence (XP_216864); it looks like our antibody should recognize it but it looks the same size as mouse and human. We would advice you to proceed with caution and let us know how you are getting on.

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