Overview

  • Product name

  • Description

    Rabbit polyclonal to UBC4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Drosophila melanogaster, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human UBC4 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab126283)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; NIH3T3; HEK293; HepG2; MCF7.
  • General notes

     This product was previously labelled as UBE2D2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab125946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2(17)KB2 antibody
    • OTTHUMP00000223475 antibody
    • OTTHUMP00000223476 antibody
    • OTTHUMP00000223477 antibody
    • OTTHUMP00000223478 antibody
    • OTTHUMP00000224375 antibody
    • PUBC 1 antibody
    • PUBC1 antibody
    • UB2D2_HUMAN antibody
    • UBC 4 antibody
    • UBC 4/5 antibody
    • UBC4 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog yeast antibody
    • UBCH 5B antibody
    • UBCH5B antibody
    • UBE2D2 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D2 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 2 antibody
    • Ubiquitin conjugating enzyme E2 D2 antibody
    • Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 2 antibody
    • Ubiquitin protein ligase D2 antibody
    • Ubiquitin-conjugating enzyme E2 D2 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 2 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody
    • Ubiquitin-protein ligase D2 antibody
    see all

Images

  • All lanes : Anti-UBC4 antibody (ab125946) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa
    Additional bands at: 102 kDa, 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References

ab125946 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab125946.
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