Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11031(B)] to UBC4 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-UBC4 antibody [EPR11031(B)] - BSA and Azide free
See all UBC4 primary antibodies
DescriptionRabbit monoclonal [EPR11031(B)] to UBC4 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human UBC4. The exact sequence is proprietary.
Database link: P62837
ab249183 is the carrier-free version of ab155088 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab249183 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as UBE2D2
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab249183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- E2(17)KB2 antibody
- OTTHUMP00000223475 antibody
- OTTHUMP00000223476 antibody
All lanes : Anti-UBC4 antibody [EPR11031(B)] (ab155088) at 1/1000 dilution
Lane 1 : MCF cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
This data was developed using ab155088, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249183 has not yet been referenced specifically in any publications.