Recombinant Anti-Ube1L antibody [EPR4269(2)] (ab133479)
![Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-484484-anti-ube1l-e1-ubiquitin-activating-enzyme-1-uba1-antibody-epr42692-western-blot.jpg "Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4269(2)] to Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Ube1L antibody [EPR4269(2)] -
Description
Rabbit monoclonal [EPR4269(2)] to Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Ube1L (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HL60, HepG2, HeLa and fetal liver lysate. GST tagged Recombinant human UBA7(Ube1L) protein. GST tagged Recombinant human UBA1 protein. IHC-P: Human urinary bladder carcinoma tissue. Flow Cyt (Intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4269(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 111 kDa.
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| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 111 kDa. |
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Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Images
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Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/1000 dilution
Lane 1 : GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 111 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-147125-anti-ube1l-antibody-epr42692-western-blot.jpg)
Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/10000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 111 kDa -
![Flow Cytometry (Intracellular) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-215920-anti-ube1l-antibody-epr42692-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)Overlay histogram showing HeLa cells stained with ab133479 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133479, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-147124-anti-ube1l-antibody-epr42692-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma tissue labelled with ab133479 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-4-benefits-of-recombinant-antibodies.png)
Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab133479 has been referenced in 1 publication.
- Cho YJ et al. Bufalin suppresses endometriosis progression by inducing pyroptosis and apoptosis. J Endocrinol 237:255-269 (2018). PubMed: 29636364
