Product nameAnti-Ube1L antibody [EPR4269(2)]
See all Ube1L primary antibodies
DescriptionRabbit monoclonal [EPR4269(2)] to Ube1L
Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Ube1L (N terminal). The exact sequence is proprietary.
- HL60, HepG2, HeLa and fetal liver lysates; Human urinary bladder carcinoma tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 111 kDa.|
|ICC/IF||1/25 - 1/50.|
|Flow Cyt||1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/10000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 111 kDa
Overlay histogram showing HeLa cells stained with ab133479 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133479, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin-embedded Human urinary bladder carcinoma tissue labelled with ab133479 at 1/100 dilution.
ab133479 has not yet been referenced specifically in any publications.