Recombinant
Anti-Ube1L antibody [EPR4269(2)] (ab133479)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Ube1L antibody [EPR4269(2)]
See all Ube1L primary antibodies -
DescriptionRabbit monoclonal [EPR4269(2)] to Ube1L
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Host speciesRabbit
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Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Ube1L (N terminal). The exact sequence is proprietary.
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Positive control
- HL60, HepG2, HeLa and fetal liver lysates; Human urinary bladder carcinoma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4269(2)
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab133479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/10000 - 1/50000. Predicted molecular weight: 111 kDa. | |
| ICC/IF | 1/25 - 1/50. | |
| Flow Cyt | 1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
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Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
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Database links
- Entrez Gene: 7318 Human
- Entrez Gene: 74153 Mouse
- Entrez Gene: 301000 Rat
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
- Unigene: 277125 Mouse
- Unigene: 98275 Rat
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Alternative names
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
see all
Images
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![Western blot - Anti-Ube1L antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/Ube1L-Primary-antibodies-ab133479-1.jpg)
Western blot - Anti-Ube1L antibody [EPR4269(2)] (ab133479)All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/10000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 111 kDa -
![Flow Cytometry - Anti-Ube1L antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/ab133479-3-ab133479FC.jpg)
Flow Cytometry - Anti-Ube1L antibody [EPR4269(2)] (ab133479)Overlay histogram showing HeLa cells stained with ab133479 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133479, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L antibody [EPR4269(2)] (ab133479)](https://www.abcam.com/ps/products/133/ab133479/Images/Ube1L-Primary-antibodies-ab133479-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L antibody [EPR4269(2)] (ab133479)Immunohistochemical analysis of paraffin-embedded Human urinary bladder carcinoma tissue labelled with ab133479 at 1/100 dilution.
Protocols
Datasheets and documents
References
ab133479 has not yet been referenced specifically in any publications.
