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  1. Link

    ube1l-antibody-epr42692-ab133479.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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RecombinantRabMAb

Recombinant Anti-Ube1L antibody [EPR4269(2)] (ab133479)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
  • Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
  • Flow Cytometry (Intracellular) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
  • Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4269(2)] to Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-Ube1L antibody [EPR4269(2)]
  • Description

    Rabbit monoclonal [EPR4269(2)] to Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Ube1L (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HL60, HepG2, HeLa and fetal liver lysate. GST tagged Recombinant human UBA7(Ube1L) protein. GST tagged Recombinant human UBA1 protein. IHC-P: Human urinary bladder carcinoma tissue. Flow Cyt (Intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4269(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E1 Ubiquitin Activating Enzymes
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Associated products

  • Alternative Versions

    • Anti-Ube1L antibody [EPR4269(2)] - BSA and Azide free (ab248515)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • HL-60 whole cell lysate (ab7914)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 111 kDa.
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

Notes
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 111 kDa.
Flow Cyt (Intra)
1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

Images

  • Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/1000 dilution

    Lane 1 : GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)
    Lane 2 : GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 111 kDa
    Observed band size: 146,166 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    Western blot - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/10000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 111 kDa

  • Flow Cytometry (Intracellular) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    Flow Cytometry (Intracellular) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)

    Overlay histogram showing HeLa cells stained with ab133479 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133479, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)

    Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma tissue labelled with ab133479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)
    Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (ab133479)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab133479? Please let us know so that we can cite the reference in this datasheet.

ab133479 has been referenced in 1 publication.

  • Cho YJ  et al. Bufalin suppresses endometriosis progression by inducing pyroptosis and apoptosis. J Endocrinol 237:255-269 (2018). PubMed: 29636364

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ube1L antibody [EPR4269(2)]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse embryonic fibroblasts)
Permeabilization
No
Specification
Mouse embryonic fibroblasts
Fixative
acetone/methanol (50/50)
Read More

Abcam user community

Verified customer

Submitted May 16 2019

Western blot abreview for Anti-Ube1L antibody [EPR4269(2)]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
15 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 16 2019

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