RecombinantRabMAb

Recombinant Anti-Ube1L antibody [EPR4269(2)] (ab133479)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4269(2)] to Ube1L
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ube1L antibody [EPR4269(2)]
    See all Ube1L primary antibodies

  • Description

    Rabbit monoclonal [EPR4269(2)] to Ube1L

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ube1L (N terminal). The exact sequence is proprietary.

  • Positive control

    • HL60, HepG2, HeLa and fetal liver lysates; Human urinary bladder carcinoma tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab133479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 111 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.

  • Tissue specificity

    Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family.
  • Information by UniProt

  • Database links

    • Alternative names

      • D8 antibody
      • MGC12713 antibody
      • UBA1, ubiquitin activating enzyme E1 homolog B antibody
      • UBA1B antibody
      • UBA7 antibody
      • UBA7, ubiquitin activating enzyme E1 antibody
      • UBA7_HUMAN antibody
      • Ube 1L antibody
      • UBE 2 antibody
      • UBE1L antibody
      • UBE2 antibody
      • Ubiquitin activating enzyme 2 antibody
      • Ubiquitin activating enzyme E1 homolog antibody
      • Ubiquitin Activating Enzyme E1 Like antibody
      • Ubiquitin activating enzyme E1 related protein antibody
      • Ubiquitin-activating enzyme 7 antibody
      • Ubiquitin-activating enzyme E1 homolog antibody
      • ubiquitin-activating enzyme-2 antibody
      • ubiquitin-like modifier activating enzyme 7 antibody
      • Ubiquitin-like modifier-activating enzyme 7 antibody
      see all

    Images

    • Western blot - Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      Western blot - Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      All lanes : Anti-Ube1L antibody [EPR4269(2)] (ab133479) at 1/10000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 111 kDa

    • Flow Cytometry - Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      Flow Cytometry - Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      Overlay histogram showing HeLa cells stained with ab133479 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133479, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L antibody [EPR4269(2)] (ab133479)

      Immunohistochemical analysis of paraffin-embedded Human urinary bladder carcinoma tissue labelled with ab133479 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Ube1L antibody [EPR4269(2)] (ab133479)
      Anti-Ube1L antibody [EPR4269(2)] (ab133479)

    References (1)

    Publishing research using ab133479? Please let us know so that we can cite the reference in this datasheet.

    ab133479 has been referenced in 1 publication.

    • Cho YJ  et al. Bufalin suppresses endometriosis progression by inducing pyroptosis and apoptosis. J Endocrinol 237:255-269 (2018). PubMed: 29636364

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ube1L antibody [EPR4269(2)]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse embryonic fibroblasts)
    Permeabilization
    No
    Specification
    Mouse embryonic fibroblasts
    Fixative
    acetone/methanol (50/50)
    Read More

    Abcam user community

    Verified customer

    Submitted May 16 2019

    Western blot abreview for Anti-Ube1L antibody [EPR4269(2)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    15 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 16 2019

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