Product nameAnti-Ube1L antibody [EPR4270]
See all Ube1L primary antibodies
DescriptionRabbit monoclonal [EPR4270] to Ube1L
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Ube1L (C terminal). The exact sequence is proprietary.
- HepG2, HL60, HeLa, Jurkat, and Human fetal liver cell lysates, HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionActivates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Tissue specificityExpressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
- Information by UniProt
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa
Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.
ab108929 has not yet been referenced specifically in any publications.