Overview

  • Product name

    Anti-Ube1L antibody [EPR4270]
    See all Ube1L primary antibodies
  • Description

    Rabbit monoclonal [EPR4270] to Ube1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Ube1L (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, HL60, HeLa, Jurkat, and Human fetal liver cell lysates, HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
    • Tissue specificity

      Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-activating E1 family.
    • Information by UniProt
    • Database links

    • Alternative names

      • D8 antibody
      • MGC12713 antibody
      • UBA1, ubiquitin activating enzyme E1 homolog B antibody
      • UBA1B antibody
      • UBA7 antibody
      • UBA7, ubiquitin activating enzyme E1 antibody
      • UBA7_HUMAN antibody
      • Ube 1L antibody
      • UBE 2 antibody
      • UBE1L antibody
      • UBE2 antibody
      • Ubiquitin activating enzyme 2 antibody
      • Ubiquitin activating enzyme E1 homolog antibody
      • Ubiquitin Activating Enzyme E1 Like antibody
      • Ubiquitin activating enzyme E1 related protein antibody
      • Ubiquitin-activating enzyme 7 antibody
      • Ubiquitin-activating enzyme E1 homolog antibody
      • ubiquitin-activating enzyme-2 antibody
      • ubiquitin-like modifier activating enzyme 7 antibody
      • Ubiquitin-like modifier-activating enzyme 7 antibody
      see all

    Images

    • All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 112 kDa

    • Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.

    References

    ab108929 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108929.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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