Overview

  • Product name

    Anti-Ube2B antibody [EPR7199]
    See all Ube2B primary antibodies
  • Description

    Rabbit monoclonal [EPR7199] to Ube2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Ube2B (UniProt: P63146).

  • Positive control

    • WB: Jurkat, HepG2, A375 and K-562 cell lysates
      IHC-P: Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Cellular localization

      Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
    • Information by UniProt
    • Database links

    • Alternative names

      • E2 17kDa antibody
      • E2 protein antibody
      • hHR6B antibody
      • HR6B antibody
      • RAD6 homolog B antibody
      • RAD6B antibody
      • UBC2 antibody
      • UBE2B antibody
      • UBE2B_HUMAN antibody
      • Ubiquitin carrier protein B antibody
      • Ubiquitin conjugating enzyme E2 B antibody
      • Ubiquitin conjugating enzyme E2B (RAD6 homolog) antibody
      • Ubiquitin conjugating enzyme E2B antibody
      • Ubiquitin protein ligase B antibody
      • Ubiquitin-conjugating enzyme E2 17 kDa antibody
      • Ubiquitin-conjugating enzyme E2 B antibody
      • Ubiquitin-conjugating enzyme E2-17 kDa antibody
      • Ubiquitin-protein ligase B antibody
      see all

    Images

    • All lanes : Anti-Ube2B antibody [EPR7199] (ab128951) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : K-562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa
      Observed band size: 17 kDa

    • ab128951, at a 1/100 dilution, staining Ube2B in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128951 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jul 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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