Product nameUbe2B overexpression 293T lysate (whole cell)
DescriptionUbe2B overexpression lysate
ab94308 is a 293T cell transfected lysate in which Human Ube2B has been transiently over-expressed using a pCMV-Ube2B plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- E2 Ubiquitin Conjugating Enzymes
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
BackgroundFunction: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.
Our Abpromise guarantee covers the use of ab94308 in the following tested applications.
|WB||Use at an assay dependent dilution.|
ab94308 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-Ube2B antibody (ab67118) at 1/500 dilution
Lane 1 : Ube2B 293T Transfected Lysate - Positive Control (ab94308)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94308 has not yet been referenced specifically in any publications.