Anti-UBE2C antibody [1F5D3] (ab201979)
Key features and details
- Mouse monoclonal [1F5D3] to UBE2C
- Suitable for: IHC-P, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-UBE2C antibody [1F5D3]
See all UBE2C primary antibodies -
Description
Mouse monoclonal [1F5D3] to UBE2C -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein corresponding to Human UBE2C aa 1 to the C-terminus.
Database link: O00762 -
Positive control
- Human liver cancer tissue and esophageal cancer tissue. UBE2C transfected HEK293 cell lysate. Recombinant human UBE2C.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1F5D3 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
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Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Post-translational
modificationsAutoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed. - Information by UniProt
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Database links
- Entrez Gene: 506962 Cow
- Entrez Gene: 11065 Human
- Entrez Gene: 68612 Mouse
- Omim: 605574 Human
- SwissProt: Q32PA5 Cow
- SwissProt: O00762 Human
- SwissProt: Q9D1C1 Mouse
- Unigene: 93002 Human
see all -
Alternative names
- Cyclin selective ubiquitin carrier protein antibody
- dJ447F3.2 antibody
- Mitotic specific ubiquitin conjugating enzyme antibody
see all
Images
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Anti-UBE2C antibody [1F5D3] (ab201979) at 1/500 dilution + recombinant UBE2C protein
Predicted band size: 20 kDa -
All lanes : Anti-UBE2C antibody [1F5D3] (ab201979) at 1/500 dilution
Lane 1 : Non-transfected HEK293 cell lysate
Lane 2 : UBE2C transfected HEK293 cell lysate
Predicted band size: 20 kDa -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues labeling UBE2C using ab201979 at 1/200 dilution followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues labeling UBE2C using ab201979 at 1/200 dilution followed by DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab201979 has been referenced in 3 publications.
- Alafate W et al. Combined elevation of AURKB and UBE2C predicts severe outcomes and therapy resistance in glioma. Pathol Res Pract 215:152557 (2019). PubMed: 31353228
- Brockmann MM et al. Neddylation regulates excitatory synaptic transmission and plasticity. Sci Rep 9:17935 (2019). PubMed: 31784571
- Ma R et al. High expression of UBE2C is associated with the aggressive progression and poor outcome of malignant glioma. Oncol Lett 11:2300-2304 (2016). PubMed: 26998166