Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE2C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human UBE2C (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Recombinant UBE2C expression in transfected HEK-293T cells, whole cell extract; NIH/3T3 whole cell extract. ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications

    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cyclin selective ubiquitin carrier protein antibody
    • dJ447F3.2 antibody
    • Mitotic specific ubiquitin conjugating enzyme antibody
    • UB E2C antibody
    • UBCH 10 antibody
    • UbcH10 antibody
    • UBE 2C antibody
    • Ube2c antibody
    • UBE2C_HUMAN antibody
    • Ubiquitin carrier protein C antibody
    • Ubiquitin carrier protein E2 C antibody
    • Ubiquitin carrier protein E2C antibody
    • Ubiquitin conjugating enzyme E2 C antibody
    • Ubiquitin conjugating enzyme E2C antibody
    • Ubiquitin protein ligase C antibody
    • Ubiquitin-conjugating enzyme E2 C antibody
    • Ubiquitin-protein ligase C antibody
    see all

Images

  • Anti-UBE2C antibody (ab226924) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 20 kDa



    12% SDS PAGE

  • A549 (human lung carcinoma cell line) cells stained for UBE2C (green) using ab226924 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-UBE2C antibody (ab226924) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) untransfected whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with UBE2C, whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 20 kDa



    12% SDS PAGE

References

ab226924 has not yet been referenced specifically in any publications.

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