Overview

  • Product name

    Anti-UBE2E2 antibody [EPR13003(B)]
    See all UBE2E2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13003(B)] to UBE2E2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96LR5

  • Positive control

    • HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ25157 antibody
      • UB2E2_HUMAN antibody
      • UBC4/5 homolog yeast antibody
      • UBCH 8 antibody
      • UbcH8 antibody
      • Ube2e2 antibody
      • Ubiquitin carrier protein E2 antibody
      • Ubiquitin conjugating enzyme E2 E2 antibody
      • Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5) antibody
      • Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2E 2 antibody
      • Ubiquitin protein ligase E2 antibody
      • Ubiquitin-conjugating enzyme E2 E2 antibody
      • Ubiquitin-protein ligase E2 antibody
      see all

    Images

    • All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 22 kDa

    References

    This product has been referenced in:

    • Gan N  et al. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol 4:134-143 (2019). Read more (PubMed: 30420781) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177485.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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