Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-UBE2E2 antibody [EPR13003(B)]
See all UBE2E2 primary antibodies -
DescriptionRabbit monoclonal [EPR13003(B)] to UBE2E2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96LR5 -
Positive control
- HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13003(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab177485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
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Database links
- Entrez Gene: 7325 Human
- Entrez Gene: 218793 Mouse
- Entrez Gene: 361013 Rat
- Omim: 602163 Human
- SwissProt: Q96LR5 Human
- SwissProt: Q91W82 Mouse
- Unigene: 475688 Human
- Unigene: 595802 Human
see all -
Alternative names
- FLJ25157 antibody
- UB2E2_HUMAN antibody
- UBC4/5 homolog yeast antibody
see all
Images
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![Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)](https://www.abcam.com/ps/products/177/ab177485/Images/ab177485-205412-ab177485.jpg)
Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
Datasheets and documents
References
ab177485 has not yet been referenced specifically in any publications.
