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RecombinantRabMAb

Recombinant Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)

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Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
  • Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13003(B)] to UBE2E2
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-UBE2E2 antibody [EPR13003(B)]
    See all UBE2E2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13003(B)] to UBE2E2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96LR5

  • Positive control

    • HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
Notes
WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ25157 antibody
    • UB2E2_HUMAN antibody
    • UBC4/5 homolog yeast antibody
    • UBCH 8 antibody
    • UbcH8 antibody
    • Ube2e2 antibody
    • Ubiquitin carrier protein E2 antibody
    • Ubiquitin conjugating enzyme E2 E2 antibody
    • Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5) antibody
    • Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2E 2 antibody
    • Ubiquitin protein ligase E2 antibody
    • Ubiquitin-conjugating enzyme E2 E2 antibody
    • Ubiquitin-protein ligase E2 antibody
    see all

Images

  • Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
    Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
    All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : LNCaP cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
    Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)

References (1)

Publishing research using ab177485? Please let us know so that we can cite the reference in this datasheet.

ab177485 has been referenced in 1 publication.

  • Gan N  et al. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol 4:134-143 (2019). PubMed: 30420781

Customer reviews and Q&As

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