RecombinantRabMAb

Recombinant Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13003(B)] to UBE2E2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-UBE2E2 antibody [EPR13003(B)]
    See all UBE2E2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13003(B)] to UBE2E2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96LR5

  • Positive control

    • HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab177485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • FLJ25157 antibody
      • UB2E2_HUMAN antibody
      • UBC4/5 homolog yeast antibody
      • UBCH 8 antibody
      • UbcH8 antibody
      • Ube2e2 antibody
      • Ubiquitin carrier protein E2 antibody
      • Ubiquitin conjugating enzyme E2 E2 antibody
      • Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5) antibody
      • Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2E 2 antibody
      • Ubiquitin protein ligase E2 antibody
      • Ubiquitin-conjugating enzyme E2 E2 antibody
      • Ubiquitin-protein ligase E2 antibody
      see all

    Images

    • Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
      Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
      All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 22 kDa

    • Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
      Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)

    References (1)

    Publishing research using ab177485? Please let us know so that we can cite the reference in this datasheet.

    ab177485 has been referenced in 1 publication.

    • Gan N  et al. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol 4:134-143 (2019). PubMed: 30420781

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