Product nameAnti-UBE2E2 antibody [EPR13003(B)]
See all UBE2E2 primary antibodies
DescriptionRabbit monoclonal [EPR13003(B)] to UBE2E2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96LR5
- HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- FLJ25157 antibody
- UB2E2_HUMAN antibody
- UBC4/5 homolog yeast antibody
All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
ab177485 has not yet been referenced specifically in any publications.