Anti-UBE2E2 antibody [EPR13003(B)] Recombinant (ab177485)

Product name

Anti-UBE2E2 antibody [EPR13003(B)]
See all UBE2E2 primary antibodies
Description

Rabbit monoclonal [EPR13003(B)] to UBE2E2
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96LR5
Positive control
- HeLa, LNCaP, 293T and Jurkat cell lysates.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13003(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab177485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination.
Pathway

Protein modification; protein ubiquitination.
Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.
Target information above from: UniProt accession Q96LR5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7325 Human
- Entrez Gene: 218793 Mouse
- Entrez Gene: 361013 Rat
- Omim: 602163 Human
- SwissProt: Q96LR5 Human
- SwissProt: Q91W82 Mouse
- Unigene: 475688 Human
- Unigene: 595802 Human
- Unigene: 23551 Mouse
- Unigene: 15203 Rat
see all
Alternative names
- FLJ25157 antibody
- UB2E2_HUMAN antibody
- UBC4/5 homolog yeast antibody
- UBCH 8 antibody
- UbcH8 antibody
- Ube2e2 antibody
- Ubiquitin carrier protein E2 antibody
- Ubiquitin conjugating enzyme E2 E2 antibody
- Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5) antibody
- Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast) antibody
- Ubiquitin conjugating enzyme E2E 2 antibody
- Ubiquitin protein ligase E2 antibody
- Ubiquitin-conjugating enzyme E2 E2 antibody
- Ubiquitin-protein ligase E2 antibody
see all

ab177485 has not yet been referenced specifically in any publications.

Publishing research using ab177485? Please let us know so that we can cite the reference in this datasheet.

Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)