Overview

  • Product name

    Anti-Ube2G1 antibody - N-terminal
    See all Ube2G1 primary antibodies
  • Description

    Rabbit polyclonal to Ube2G1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 3-33 of Human Ube2G1 (NP_003333).

  • Positive control

    • Mouse heart tissue lysate; 293 cell lysate transfected with the Ube2G1 gene; Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab135508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 20 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity

    Widely expressed, mainly in skeletal muscle.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links

  • Alternative names

    • E217K antibody
    • UB2G1_HUMAN antibody
    • UBC 7 antibody
    • UBC7 antibody
    • UBC7 homolog yeast antibody
    • UBE 2G antibody
    • UBE2G antibody
    • Ube2g1 antibody
    • Ubiquitin carrier protein G1 antibody
    • Ubiquitin conjugating enzyme E2 G1 antibody
    • Ubiquitin conjugating enzyme E2G 1 (homologous to C. elegans UBC7) antibody
    • Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog C. elegans) antibody
    • Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2G 1 antibody
    • Ubiquitin protein ligase G1 antibody
    • Ubiquitin-conjugating enzyme E2 G1 antibody
    • Ubiquitin-protein ligase G1 antibody
    see all

Images

  • Anti-Ube2G1 antibody - N-terminal (ab135508) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size: 20 kDa

  • All lanes : Anti-Ube2G1 antibody - N-terminal (ab135508) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the Ube2G1 gene.

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 20 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling Ube2G1 with ab135508 at 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.

References

ab135508 has not yet been referenced specifically in any publications.

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