Product nameUbe2G1 overexpression 293T lysate (whole cell)
See all Ube2G1 lysates
ab94306 is a 293T cell transfected lysate in which Human Ube2G1 has been transiently over-expressed using a pCMV-Ube2G1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Tissue specificity: Widely expressed, mainly in skeletal muscle. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.
Our Abpromise guarantee covers the use of ab94306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94306 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-Ube2G1 antibody (ab58256) at 1/500 dilution
Lane 1 :
Ube2G1 overexpression 293T lysate (whole cell) (ab94306)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94306 has not yet been referenced specifically in any publications.